Single Synapse Indicators of Impaired Glutamate Clearance Derived from Fast iGlu _u Imaging of Cortical Afferents in the Striatum of Normal and Huntington (Q175) Mice.

ABSTRACT: Changes in the balance between glutamate (Glu) release and uptake may stimulate synaptic reorganization and even synapse loss. In the case of neurodegeneration, a mismatch between astroglial Glu uptake and presynaptic Glu release could be detected if both parameters were assessed independently and at a single-synapse level. This has now become possible due to a new imaging assay with the genetically encoded ultrafast Glu sensor iGlu _u We report findings from individual corticostriatal synapses in acute slices prepared from mice of either sex that were >1 year of age. Contrasting patterns of short-term plasticity and a size criterion identified two classes of terminals, presumably corresponding to the previously defined IT (intratelencephalic) and PT (pyramidal tract) syna

SUBMITTER: Dvorzhak A 

PROVIDER: S-EPMC6520508 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature