Ultra-deep, long-read nanopore sequencing of mock microbial community standards.

ABSTRACT:

Background

Long sequencing reads are information-rich: aiding de novo assembly and reference mapping, and consequently have great potential for the study of microbial communities. However, the best approaches for analysis of long-read metagenomic data are unknown. Additionally, rigorous evaluation of bioinformatics tools is hindered by a lack of long-read data from validated samples with known composition.

Findings

We sequenced 2 commercially available mock communities containing 10 microbial species (ZymoBIOMICS Microbial Community Standards) with Oxford Nanopore GridION and PromethION. Both communities and the 10 individual species isolates were also sequenced with Illumina technology. We generated 14 and 16 gigabase pairs from 2 GridION flowcells and 150 and 153 gigabase

SUBMITTER: Nicholls SM 

PROVIDER: S-EPMC6520541 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature