Centrosomal Actin Assembly Is Required for Proper Mitotic Spindle Formation and Chromosome Congression.

ABSTRACT: Cytoskeletal cross talk between actin filaments and microtubules is a common mechanism governing the assembly of cellular structures, i.e., during filopodia formation or cilia organization. However, potential actin-microtubule interactions during mammalian cell divisions are less well understood. At mitotic entry, centrosomes propagate the formation of the mitotic spindle, thereby aligning individual chromosomes to the metaphase plate, a process coined chromosome congression. Here, we identify actin filament assembly spatially defined at centrosomes contemporaneously with spindle microtubules forming during prometaphase. We show that pharmacological Arp2/3 complex inhibition as well as overexpression of the Arp2/3 complex inhibitory protein Arpin decreased spindle actin. As a consequence,

SUBMITTER: Plessner M 

PROVIDER: S-EPMC6520610 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature