Structural Insights to the Heterotetrameric Interaction between the Vibrio parahaemolyticus PirA^vp and PirB^vp Toxins and Activation of the Cry-Like Pore-Forming Domain.

ABSTRACT: Acute hepatopancreatic necrosis disease (AHPND) is a newly emergent penaeid shrimp disease which can cause 70-100% mortality in Penaeus vannamei and Penaeus monodon, and has resulted in enormous economic losses since its appearance. AHPND is caused by the specific strains of Vibrio parahaemolyticus that harbor the pVA1 plasmid and express PirA^vp and PirB^vp toxins. These two toxins have been reported to form a binary complex. When both are present, they lead to the death of shrimp epithelial cells in the hepatopancreas and cause the typical histological symptoms of AHPND. However, the binding mode of PirA^vp and PirB^vp has not yet been determined. Here, we used isothermal titration calorimetry (ITC)

SUBMITTER: Lin SJ 

PROVIDER: S-EPMC6520838 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature