Dataset Information

Three Polymethoxyflavones Purified from Ougan (Citrus reticulata Cv. Suavissima) Inhibited LPS-Induced NO Elevation in the Neuroglia BV-2 Cell Line via the JAK2/STAT3 Pathway.

ABSTRACT: In order to establish an efficient method for separation of polymethoxyflavones (PMFs) and explore the anti-inflammatory mechanism of PMF monomers, a citrus variety rich in PMFs, Ougan (Citrus reticulata cv. Suavissima), was selected, and three monomers, including nobiletin, tangeretin, and 5-demethylnobiletin, were purified by ultrasonic-assisted extraction, solid phase extraction, and high-speed countercurrent chromatography separation. UPLC-MS was used to identify the three monomers. UPLC determined purities of 99.87% to nobiletin, 99.76% to tangeretin, and 98.75% to 5-demethylnobiletin with the standard curve method. A lipopolysaccharide (LPS)-induced NO releasing model was performed in the mouse microglia BV-2 cell line. Results illustrated that PMF monomers inhibited the NO release and the inflammation-related cytokines, including IL-1?, IL-6, and TNF? elevation. QRT-PCR revealed that PMFs alleviated LPS-induced upregulation of iNOS, IL-6, JAK2, TNF?, IL-1?, and NF-?B and LPS-induced downregulation of I?B?, while they did not affect TLR1, TLR2, TLR4, and TLR6. STAT3 expression was repressed by tangeretin and 5-demethylnobiletin, but not by nobiletin. Western blot assay also showed a suppression of expression and phosphorylation of JAK2 by all three PMF monomers, while STAT3 phosphorylation was restrained by tangeretin and 5-demethylnobiletin. The mechanism was primarily verified by the JAK2 inhibitor Ruxolitinib and the STAT3 inhibitor Stattic.

SUBMITTER: Wang Y

PROVIDER: S-EPMC6521056 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Publications

Three Polymethoxyflavones Purified from Ougan (Citrus reticulata Cv. Suavissima) Inhibited LPS-Induced NO Elevation in the Neuroglia BV-2 Cell Line via the JAK2/STAT3 Pathway.

Wang Yue Y Zang Wenjing W Ji Shiyu S Cao Jinping J Sun Chongde C

Nutrients 20190405 4

In order to establish an efficient method for separation of polymethoxyflavones (PMFs) and explore the anti-inflammatory mechanism of PMF monomers, a citrus variety rich in PMFs, Ougan (Citrus reticulata cv. Suavissima), was selected, and three monomers, including nobiletin, tangeretin, and 5-demethylnobiletin, were purified by ultrasonic-assisted extraction, solid phase extraction, and high-speed countercurrent chromatography separation. UPLC-MS was used to identify the three mono ...[more]

PMID: 30959824

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Project description:Rapid urbanization and increasing land scarcity have made urban agriculture and efficient space utilization critical directions in modern agriculture. Ougan, a fruit tree valued for both its economic and ecological benefits, holds significant promise for dwarfing cultivation techniques. In this study, a root-irrigation method was used to apply paclobutrazol at various concentrations (200, 500, 1000, 1500, and 2000 mg/L) to Ougan seedlings, with a control group for comparison. Growth parameters include an average daily increase of plant height, stem girth, new branches, and new branch girth, as well as physiological indices such as leaf SPAD values, leaf nitrogen content, net photosynthetic rate, stomatal conductance, intercellular CO2 concentration, and transpiration rate, were measured during both spring and summer growth periods. The results demonstrate that PBZ exerts a distinct concentration-dependent regulatory effect on Ougan growth: higher concentrations significantly inhibited plant height while promoting increases in stem diameter, with several parameters exhibiting a unimodal response. Short-term (spring) PBZ application enhanced certain photosynthetic parameters, such as net photosynthetic rate and stomatal conductance; however, prolonged exposure (summer) resulted in a decline in photosynthetic efficiency and overall leaf physiological status. Through comprehensive evaluation using principal component analysis and PLS-SEM, the 500 mg/L PBZ treatment was identified as achieving the optimal balance between growth inhibition and the maintenance of photosynthetic and nutritional status, closely approximating the ideal dwarfing effect. This study elucidates the complex regulatory effects of PBZ on the growth, photosynthesis, and carbon assimilation of Ougan through natural climate, providing robust technical parameters and theoretical support for future dwarf cultivation practices. These findings facilitate the development of dwarf fruit trees into bonsai vegetation, demonstrating significant horticultural application potential.

Project description:Citrus is a globally consumed fruit with great popularity. Mandarin (Citrus reticulata cv. 'Shatangju') is a local variety, and its planting area and yield are the greatest regarding fruit tree planting in Guangdong Province, China. However, its resistance to Huanglongbing (HLB) is weak. After infection by HLB, the fruits cannot develop normally. In this study, four kinds of fruits were classified as HBG, XQG, ZQG, and DHG, according to the color of their peels. The metabolomes of the three abnormally colored groups (HBG, XQG, and ZQG) and the normally colored group (DHG) were compared using a UPLC-QQQ-MS-based metabolomics approach. In total, 913 metabolites were identified and classified into 23 different categories, including phenylpropanoids and flavonoids; among them, 215 (HBG, 177; XQG, 124; and ZQG, 62) metabolites showed differential accumulation in the three comparison groups (HBG/XQG/ZQG versus DHG). A total of 2 unique metabolites, O-caffeoyl maltotriose and myricetin were detected only in DHG samples. When comparing HBG with DHG, there were 109 decreased and 68 increased metabolites; comparing XQG with DHG, there were 88 decreased and 36 increased metabolites; comparing ZQG with DHG, 41 metabolites were decreased, and 21 metabolites were increased. Metabolic pathway enrichment analysis of these differential metabolites showed significant enrichment of the "phenylpropanoid biosynthesis" pathway in all comparison groups. The hierarchical cluster analysis of the differential metabolites of the four groups showed a clear grouping patterns. The relative contents of three phenylpropanoids, four flavonoids, two alkaloids, one anthocyanin, and two other metabolites were significantly different between each comparison group. This study might provide fundamental insight for the isolation and identification of functional compounds from the peels of citrus fruit infected with HLB and for in-depth research on the effect of HLB on the formation of fruits pigment and the development of HLB-resistant citrus varieties.

Dataset Information

Three Polymethoxyflavones Purified from Ougan (Citrus reticulata Cv. Suavissima) Inhibited LPS-Induced NO Elevation in the Neuroglia BV-2 Cell Line via the JAK2/STAT3 Pathway.

Publications

Three Polymethoxyflavones Purified from Ougan (<i>Citrus</i> <i>reticulata</i> Cv. <i>Suavissima</i>) Inhibited LPS-Induced NO Elevation in the Neuroglia BV-2 Cell Line via the JAK2/STAT3 Pathway.

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