CesH Represses Cereulide Synthesis as an Alpha/Beta Fold Hydrolase in Bacillus cereus.

ABSTRACT: Cereulide is notorious as a heat-stable emetic toxin produced by Bacillus cereus and glucose is supposed to be an ingredient supporting its formation. This study showed that glucose addition benefited on cell growth and the early transcription of genes involved in substrate accumulation and toxin synthesis, but it played a negative role in the final production of cereulide. Meanwhile, a lasting enhancement of cesH transcription was observed with the addition of glucose. Moreover, the cereulide production in ΔcesH was obviously higher than that in the wild type. This indicates that CesH has a repression effect on cereulide production. Bioinformatics analysis revealed that CesH was an alpha/beta hydrolase that probably associated with the cell membrane, which was verifie

SUBMITTER: Tian S 

PROVIDER: S-EPMC6521208 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature