Altered CSF Proteomic Profiling of Paediatric Acute Lymphocytic Leukemia Patients with CNS Infiltration.

ABSTRACT:

Background

For childhood acute lymphocytic leukemia (ALL), central nervous system leukemia (CNSL) is still the main reason of treatment failure. Changes of cerebrospinal fluid (CSF) proteome are deemed to occur after intrathecal chemotherapy.

Objective

To find critical CSF biomarkers, which could be utilized to increase diagnostic and prognostic accuracy of CNSL.

Methods

We performed proteomic profiling of CSF before and after the treatment of six sporadic paediatric patients diagnosed as ALL with central nervous system (CNS) involvement. CSF samples were properly processed and analyzed through the use of label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Results

Among identified 428 unique proteins in all CSF samples, we quantified 10 altere

SUBMITTER: Mo F 

PROVIDER: S-EPMC6521476 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature