Genetic diversity of avocado (Persea americana Mill.) germplasm using pooled sequencing.

ABSTRACT:

Background

Discovering a genome-wide set of avocado (Persea americana Mill.) single nucleotide polymorphisms and characterizing the diversity of germplasm collection is a powerful tool for breeding. However, discovery is a costly process, due to loss of loci that are proven to be non-informative when genotyping the germplasm.

Results

Our study on a collection of 100 accessions comprised the three race types, Guatemalan, Mexican, and West Indian. To increase the chances of discovering polymorphic loci, three pools of genomic DNA, one from each race, were sequenced and the reads were aligned to a reference transcriptome. In total, 507,917 polymorphic loci were identified in the entire collection. Of these, 345,617 were observed in all three pools, 117,692 in two pools, 44,552

SUBMITTER: Rubinstein M 

PROVIDER: S-EPMC6521498 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature