A Chemogenomic Screening Platform Used to Identify Chemotypes Perturbing HSP90 Pathways.

ABSTRACT: Compounds that modulate the heat shock protein (HSP) network have potential in a broad range of research applications and diseases. A yeast-based liquid culture assay that measured time-dependent turbidity enabled the high-throughput screening of different Saccharomyces cerevisae strains to identify HSP modulators with unique molecular mechanisms. A focused set of four strains, with differing sensitivities to Hsp90 inhibitors, was used to screen a compound library of 3680 compounds. Computed turbidity curve functions were used to classify strain responses and sensitivity to chemical effects across the compound library. Filtering based on single-strain selectivity identified nine compounds as potential heat shock modulators, including the known Hsp90 inhibitor macbecin. Haploid yeast deleti

SUBMITTER: Thomas FM 

PROVIDER: S-EPMC6521697 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature