De Novo Glycan Sequencing by Electronic Excitation Dissociation and Fixed-Charge Derivatization.

ABSTRACT: Detailed glycan structural characterization is frequently achieved by collisionally activated dissociation (CAD) based sequential tandem mass spectrometry (MS ⁿ) analysis of permethylated glycans. However, it is challenging to implement MS ⁿ ( n > 2) during online glycan separation, and this has limited its application to analysis of complex glycan mixtures from biological samples. Further, permethylation can reduce liquid chromatographic (LC) resolution of isomeric glycans. Here, we studied the electronic excitation dissociation (EED) fragmentation behavior of native glycans with a reducing-end fixed charge tag and identified key spectral features that are useful for topology and linkage determination. We also developed a de novo glycan sequencing software that showe

SUBMITTER: Tang Y 

PROVIDER: S-EPMC6521716 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature