Proliferation tracing with single-cell mass cytometry optimizes generation of stem cell memory-like T cells.

ABSTRACT: Selective differentiation of naive T cells into multipotent T cells is of great interest clinically for the generation of cell-based cancer immunotherapies. Cellular differentiation depends crucially on division state and time. Here we adapt a dye dilution assay for tracking cell proliferative history through mass cytometry and uncouple division, time and regulatory protein expression in single naive human T cells during their activation and expansion in a complex ex vivo milieu. Using 23 markers, we defined groups of proteins controlled predominantly by division state or time and found that undivided cells account for the majority of phenotypic diversity. We next built a map of cell state changes during naive T-cell expansion. By examining cell signaling on this map, we rationally selecte

SUBMITTER: Good Z 

PROVIDER: S-EPMC6521980 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature