Complete genome sequencing and evolutionary analysis of HCV subtype 6xg from IDUs in Yunnan, China.

ABSTRACT:

Background

HCV genotype 6 (HCV-6) typically circulates in Southeast Asia and exhibits the highest genetic diversity among the eight HCV genotypes. In our previous work, a group of HCV-6 sequences was not clearly classified. Here, we further characterized this HCV-6 variant and analyzed the evolutionary history of the enlarged HCV-6 family.

Methods

Blood samples from eight HCV seropositive samples collected from intravenous drug users (IDUs) in 2014 in Yunnan Province, China. The full-length HCV genome sequences were amplified by using reverse transcription PCR followed by DNA sequencing and phylogenetic analysis. Bayesian evolutionary analysis was performed with the complete coding region sequences of subtype 6a-6xh.

Results

The eight genomes had the same coding regio

SUBMITTER: Chen M 

PROVIDER: S-EPMC6522032 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature