Human colon function ex vivo: Dependence on oxygen and sensitivity to antibiotic.

ABSTRACT:

Background

Human intestines contain a heterogeneous collection of cells that include immune, neural and epithelial elements interacting in a highly complex physiology that is challenging to maintain ex vivo. There is an extreme oxygen gradient across the intestinal wall due in part to microbiota in the lumen and close to the gut wall, which complicates the design of tissue culture systems. The current study established the use of an organotypic slice model of human intestinal tissue derived from colonoscopy biopsies to study host-microbial interactions after antibiotic treatment, and the influence of oxygen concentration on gut wall function.

Methods

Organotypic slices from human colon biopsies collected during routine colonoscopy provided three-dimensional environments that

SUBMITTER: Schwerdtfeger LA 

PROVIDER: S-EPMC6522050 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature