Refining wet lab experiments with in silico searches: A rational quest for diagnostic peptides in visceral leishmaniasis.

ABSTRACT:

Background

The search for diagnostic biomarkers has been profiting from a growing number of high quality sequenced genomes and freely available bioinformatic tools. These can be combined with wet lab experiments for a rational search. Improved, point-of-care diagnostic tests for visceral leishmaniasis (VL), early case detection and surveillance are required. Previous investigations demonstrated the potential of IgG1 as a biomarker for monitoring clinical status in rapid diagnostic tests (RDTs), although using a crude lysate antigen (CLA) as capturing antigen. Replacing the CLA by specific antigens would lead to more robust RDTs.

Methodology

Immunoblots revealed L. donovani protein bands detected by IgG1 from VL patients. Upon confident identification of these antigens by mas

SUBMITTER: Bremer Hinckel BC 

PROVIDER: S-EPMC6522066 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature