A single-cell molecular map of mouse gastrulation and early organogenesis.

ABSTRACT: Across the animal kingdom, gastrulation represents a key developmental event during which embryonic pluripotent cells diversify into lineage-specific precursors that will generate the adult organism. Here we report the transcriptional profiles of 116,312 single cells from mouse embryos collected at nine sequential time points ranging from 6.5 to 8.5 days post-fertilization. We construct a molecular map of cellular differentiation from pluripotency towards all major embryonic lineages, and explore the complex events involved in the convergence of visceral and primitive streak-derived endoderm. Furthermore, we use single-cell profiling to show that Tal1^-/- chimeric embryos display defects in early mesoderm diversification, and we thus demonstrate how combining temporal and transcr

SUBMITTER: Pijuan-Sala B 

PROVIDER: S-EPMC6522369 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature