Simultaneous polychromatic flow cytometric detection of multiple forms of regulated cell death.

ABSTRACT: Currently the study of Regulated Cell Death (RCD) processes is limited to the use of lysed cell populations for Western blot analysis of each separate RCD process. We have previously shown that intracellular antigen flow cytometric analysis of RIP3, Caspase-3 and cell viability dye allowed the determination of levels of apoptosis (Caspase-3^+ ve/RIP3^- ve), necroptosis (RIP3^Hi + ve/Caspase-3^- ve) and RIP1-dependent apoptosis (Caspase-3^+ ve/RIP3^+ ve) in a single Jurkat cell population. The addition of more intracellular markers allows the determination of the incidence of parthanatos (PARP), DNA Damage Response (DDR, H2AX), H2AX hyper-activation of PARP (H2AX/PARP) autophagy (LC3B) and ER stress (PERK), thus allowing the identi

SUBMITTER: Bergamaschi D 

PROVIDER: S-EPMC6522464 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature