Different effects of partial pressure in a high-pressure gaseous mixture of carbon monoxide and oxygen for rat heart preservation.

ABSTRACT: We maintained the function of an extracted rat heart after 24-48 h preservation in a high-pressure gaseous mixture of carbon monoxide (CO) and oxygen (O₂). Here, we assessed the effects of different partial pressures of hyperbaric CO and O₂ for 24-48 h at 4 °C on rat heart preservation and compared conditions including immersion in University of Wisconsin solution. Preserved hearts were transplanted into recipient rats via heterotopic cervical heart transplantation for in vivo evaluation and perfused using the Langendorff system for ex vivo evaluation. The survival rate of transplanted hearts was 100% at postoperative day 7 in the CO + O₂ (PCO:PO₂ = 1.5:2.0 atm) group but only 33% in the CO + O₂ (PCO:PO₂ = 2.0:1.5 atm) group

SUBMITTER: Hatayama N 

PROVIDER: S-EPMC6522590 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature