Drying Enhances Signal Intensities for Global GC⁻MS Metabolomics.

ABSTRACT: We report here that a straightforward change of the standard derivatization procedure for GC⁻MS metabolomics is leading to a strong increase in metabolite signal intensity. Drying samples between methoxymation and trimethylsilylation significantly increased signals by two- to tenfold in extracts of yeast cells, plant and animal tissue, and human urine. This easy step reduces the cost of sample material and the need for expensive new hardware.

SUBMITTER: Liebeke M 

PROVIDER: S-EPMC6523563 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature