Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus.

ABSTRACT: Fungi that infect plants, animals or humans pose a serious threat to human health and food security. Antifungal proteins (AFPs) secreted by filamentous fungi are promising biomolecules that could be used to develop new antifungal therapies in medicine and agriculture. They are small highly stable proteins with specific potent activity against fungal pathogens. However, their exploitation requires efficient, sustainable and safe production systems. Here, we report the development of an easy-to-use, open access viral vector based on Tobacco mosaic virus (TMV). This new system allows the fast and efficient assembly of the open reading frames of interest in small intermediate entry plasmids using the Gibson reaction. The manipulated TMV fragments are then transferred to the infectious clone by

SUBMITTER: Shi X 

PROVIDER: S-EPMC6523586 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature