Next-Generation Sequencing Enables Spatiotemporal Resolution of Human Centromere Replication Timing.

ABSTRACT: Centromeres serve a critical function in preserving genome integrity across sequential cell divisions, by mediating symmetric chromosome segregation. The repetitive, heterochromatic nature of centromeres is thought to be inhibitory to DNA replication, but has also led to their underrepresentation in human reference genome assemblies. Consequently, centromeres have been excluded from genomic replication timing analyses, leaving their time of replication unresolved. However, the most recent human reference genome, hg38, included models of centromere sequences. To establish the experimental requirements for achieving replication timing profiles for centromeres, we sequenced G₁- and S-phase cells from five human cell lines, and aligned the sequence reads to hg38. We were able to infer DNA repl

SUBMITTER: Massey DJ 

PROVIDER: S-EPMC6523654 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature