Perinatal Lead (Pb) Exposure and Cortical Neuron-Specific DNA Methylation in Male Mice.

ABSTRACT: : Lead (Pb) exposure is associated with a wide range of neurological deficits. Environmental exposures may impact epigenetic changes, such as DNA methylation, and can affect neurodevelopmental outcomes over the life-course. Mating mice were obtained from a genetically invariant C57BL/6J background agouti viable yellow A^vy strain. Virgin dams (a/a) were randomly assigned 0 ppm (control), 2.1 ppm (low), or 32 ppm (high) Pb-acetate water two weeks prior to mating with male mice (A^vy/a), and this continued through weaning. At age 10 months, cortex neuronal nuclei were separated with NeuN⁺ antibodies in male mice to investigate neuron-specific genome-wide promoter DNA methylation using the Roche NimbleGen Mouse 3x720K CpG Island Pr

SUBMITTER: Dou JF 

PROVIDER: S-EPMC6523909 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature