The ubiquitin ligase CHIP modulates cellular behaviors of gastric cancer cells by regulating TRAF2.

ABSTRACT:

Background

CHIP is an E3 ubiquitin ligase that plays contrast roles in diverse human malignancies, depending on its targets. To date, the mechanisms underlying the function of CHIP in gastric cancer remains unclear. Here, we aim to further clarify the effects of CHIP on the development and progression of gastric cancer and explore its potential target.

Methods

Stably transfected CHIP-shRNA and TRAF2-shRNA AGS gastric cancer cell lines were established using Lipofectamine 2000. Cell growth was measured by an xCelligence real-time monitoring system and colony formation assay. Cell proliferation was detected using CCK-8, Ki-67, or CFSE assays. Apoptosis was detected by TUNEL assay or Annexin V/PI-staining followed by flow cytometric analysis. Cell cycle distribution was detecte

SUBMITTER: Dai H 

PROVIDER: S-EPMC6524225 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature