Probing N-glycoprotein microheterogeneity by lectin affinity purification-mass spectrometry analysis.

ABSTRACT: Lectins are carbohydrate binding proteins that recognize specific epitopes present on target glycoproteins. Changes in lectin-reactive carbohydrate repertoires are related to many biological signaling pathways and recognized as hallmarks of several pathological processes. Consequently, lectins are valuable probes, commonly used for examining glycoprotein structural and functional microheterogeneity. However, the molecular interactions between a given lectin and its preferred glycoproteoforms are largely unknown due to the inherent complexity and limitations of methods used to investigate intact glycoproteins. Here, we apply a lectin-affinity purification procedure coupled with native mass spectrometry to characterize lectin-reactive glycoproteoforms at the intact protein level. We investig

SUBMITTER: Wu D 

PROVIDER: S-EPMC6524569 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature