Moments reconstruction and local dynamic range compression of high order superresolution optical fluctuation imaging.

ABSTRACT: Super-resolution optical fluctuation imaging (SOFI) offers a simple and affordable alternative to other super-resolution (SR) imaging techniques. The theoretical resolution enhancement of SOFI scales linearly with the order of cumulants, while the imaging conditions exhibit less photo-toxicity to the living samples as compared to other SR methods. High order SOFI could, therefore, be a method of choice for dynamic live cell imaging. However, due to the cusp-artifacts and dynamic range expansion of pixel intensities, this promise has not been materialized as of yet. Here we investigated and compared high order moments vs. high order cumulant SOFI reconstructions. We demonstrate that even-order moments reconstructions are intrinsically free of cusp artifacts, allowing for a subsequent deconv

SUBMITTER: Yi X 

PROVIDER: S-EPMC6524576 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature