Context-Dependent Impact of RAS Oncogene Expression on Cellular Reprogramming to Pluripotency.

ABSTRACT: Induction of pluripotency in somatic cells with defined genetic factors has been successfully used to investigate the mechanisms of disease initiation and progression. Cellular reprogramming and oncogenic transformation share common features; both involve undergoing a dramatic change in cell identity, and immortalization is a key step for cancer progression that enhances reprogramming. However, there are very few examples of complete successful reprogramming of tumor cells. Here we address the effect of expressing an active oncogene, RAS, on the process of reprogramming and found that, while combined expression with reprogramming factors enhanced dedifferentiation, expression within the context of neoplastic transformation impaired reprogramming. RAS induces expression changes that promote

SUBMITTER: Ferreiros A 

PROVIDER: S-EPMC6524732 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature