Assessing the pentose phosphate pathway using [2, 3-¹³ C₂ ]glucose.

ABSTRACT: The pentose phosphate pathway (PPP) is essential for reductive biosynthesis, antioxidant processes and nucleotide production. Common tracers such as [1,2-¹³ C₂ ]glucose rely on detection of ¹³ C in lactate and require assumptions to correct natural ¹³ C abundance. Here, we introduce a novel and specific tracer of the PPP, [2,3-¹³ C₂ ]glucose. ¹³ C NMR analysis of the resulting isotopomers is informative because [1,2-¹³ C₂ ]lactate arises from glycolysis and [2,3-¹³ C₂ ]lactate arises exclusively through the PPP. A correction for natural abundance is unnecessary. In rats receiving [2,3-¹³ C₂ ]glucose, the PPP was more active in the fed versus fasted

SUBMITTER: Lee MH 

PROVIDER: S-EPMC6525052 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature