GFP fluorescence peak fraction analysis based nanothermometer for the assessment of exothermal mitochondria activity in live cells.

ABSTRACT: Nanothermometry methods with intracellular sensitivities have the potential to make important contributions to fundamental cell biology and medical fields, as temperature is a relevant physical parameter for molecular reactions to occur inside the cells and changes of local temperature are well identified therapeutic strategies. Here we show how the GFP can be used to assess temperature-based on a novel fluorescence peak fraction method. Further, we use standard GFP transfection reagents to assess temperature intracellularly in HeLa cells expressing GFP in the mitochondria. High thermal resolution and sensitivity of around 0.26% °C^-1 and 2.5% °C^-1, were achieved for wt-GFP in solution and emGFP-Mito within the cell, respectively. We demonstrate that the GFP-based nano

SUBMITTER: Savchuk OA 

PROVIDER: S-EPMC6525231 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature