A high-throughput amplicon-based method for estimating outcrossing rates.

ABSTRACT:

Background

The outcrossing rate is a key determinant of the population-genetic structure of species and their long-term evolutionary trajectories. However, determining the outcrossing rate using current methods based on PCR-genotyping individual offspring of focal plants for multiple polymorphic markers is laborious and time-consuming.

Results

We have developed an amplicon-based, high-throughput enabled method for estimating the outcrossing rate and have applied this to an example of scented versus non-scented Capsella (Shepherd's Purse) genotypes. Our results show that the method is able to robustly capture differences in outcrossing rates. They also highlight potential biases in the estimates resulting from differential haplotype sharing of the focal plants with the

SUBMITTER: Jantzen F 

PROVIDER: S-EPMC6525360 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature