Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Correction for Helbert et al., Discovery of novel carbohydrate-active enzymes through the rational exploration of the protein sequences space.

ABSTRACT:

SUBMITTER:

PROVIDER: S-EPMC6525495 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Publications

Correction for Helbert et al., Discovery of novel carbohydrate-active enzymes through the rational exploration of the protein sequences space.

Proceedings of the National Academy of Sciences of the United States of America 20190506 20

PMID: 31061113

Similar Datasets

Discovery of novel carbohydrate-active enzymes through the rational exploration of the protein sequences space.

Project description:Over the last two decades, the number of gene/protein sequences gleaned from sequencing projects of individual genomes and environmental DNA has grown exponentially. Only a tiny fraction of these predicted proteins has been experimentally characterized, and the function of most proteins remains hypothetical or only predicted based on sequence similarity. Despite the development of postgenomic methods, such as transcriptomics, proteomics, and metabolomics, the assignment of function to protein sequences remains one of the main challenges in modern biology. As in all classes of proteins, the growing number of predicted carbohydrate-active enzymes (CAZymes) has not been accompanied by a systematic and accurate attribution of function. Taking advantage of the CAZy database, which groups CAZymes into families and subfamilies based on amino acid similarities, we recombinantly produced 564 proteins selected from subfamilies without any biochemically characterized representatives, from distant relatives of characterized enzymes and from nonclassified proteins that show little similarity with known CAZymes. Screening these proteins for activity on a wide collection of carbohydrate substrates led to the discovery of 13 CAZyme families (two of which were also discovered by others during the course of our work), revealed three previously unknown substrate specificities, and assigned a function to 25 subfamilies.

| S-EPMC6442616 | biostudies-literature

Correction for Clancy et al., Draft Genome Sequences of Helicobacter pylori Strains 17874 and P79.

Project description: Not available

| S-EPMC4907115 | biostudies-literature

Correction for Johnson et al., "Complete Genome Sequences for 35 Biothreat Assay-Relevant Bacillus Species".

Project description: Not available

| S-EPMC5773713 | biostudies-literature

Correction for Sun et al., “Large-Scale Detection of Telomeric Motif Sequences in Genomic Data Using TelFinder”

Project description: Not available

| S-EPMC10715131 | biostudies-literature

Correction for Tao et al., Lsh, chromatin remodeling family member, modulates genome-wide cytosine methylation patterns at nonrepeat sequences

Project description: Not available