Defining the core essential genome of Pseudomonas aeruginosa.

ABSTRACT: Genomics offered the promise of transforming antibiotic discovery by revealing many new essential genes as good targets, but the results fell short of the promise. While numerous factors contributed to the disappointing yield, one factor was that essential genes for a bacterial species were often defined based on a single or limited number of strains grown under a single or limited number of in vitro laboratory conditions. In fact, the essentiality of a gene can depend on both the genetic background and growth condition. We thus developed a strategy for more rigorously defining the core essential genome of a bacterial species by studying many pathogen strains and growth conditions. We assessed how many strains must be examined to converge on a set of core essential genes for a species. We

SUBMITTER: Poulsen BE 

PROVIDER: S-EPMC6525520 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature