Effect of cacao bean quality grade on cacao quality evaluation by cut test and correlations with free amino acids and polyphenols profiles.
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ABSTRACT: Cacao fermentation induces biochemical changes in the beans that lead to different cacao grades such as slaty (unfermented), violet (under fermented) and brown (fully fermented) beans. This grade heterogeneity in a sample set can impact the quality of the end-products. In the present study, amino acids and polyphenol contents of slaty, violet and brown beans were evaluated. Free amino acids were derivatized from o-phthalaldehyde and detected with a fluorescence spectrophotometer coupled with a reverse phase HPLC system. Polyphenols were analysed at 280 nm by HPLC using a Photodiode Array Detector. Amino acids content in the violet beans were significantly higher (11,165 ± 4281 mg kg-1 fat free dry material, ffdm) than that of the slaty beans (4304.5 ± 1927.6 mg kg-1<
SUBMITTER: Tchouatcheu GAN
PROVIDER: S-EPMC6525676 | biostudies-literature | 2019 May
REPOSITORIES: biostudies-literature
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