Enhanced endoxylanase production by Myceliophthora thermophila with applicability in saccharification of agricultural substrates.

ABSTRACT: The production of enzymes by solid-state fermentation is an interesting process and currently used worldwide as it can be carried out in solid matrix in absence of free water. In present study, Myceliophthora thermophila BJTLRMDU3 produced high titres of endoxylanase (890.55 U/g DR, dry residue) using 5 g rice straw at pH 7.0 and at 45 °C with 1:7 (w/v) solid-to-moisture ratio with inoculum rate of 12 × 106 spores/ml after 4 days in solid-state fermentation. High enzyme titre was produced after moistening the rice straw with solution containing ammonium sulphate (0.4%), K2HPO4 (1.0%), MgSO4·7H2O (0.3%), FeSO4·7H2O (0.03%) and CaCl2 (0.03%). Addition of sucrose (2% w/v) and ammonium nitrate (2% w/v) further enhanced the endoxylanase production. A high endoxylanase production was achieved at

SUBMITTER: Dahiya S 

PROVIDER: S-EPMC6525679 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature