Identification of Macrophage Genotype and Key Biological Pathways in Circulating Angiogenic Cell Transcriptome.

ABSTRACT:

Background

Circulating angiogenic cells (CAC) have been identified as important regulators of vascular biology. However, there is still considerable debate about the genotype and function of CAC.

Methods and results

Data from publicly available gene expression data sets were used to analyse the transcriptome of in vitro cultured CAC (CAC_iv). Genes and pathways of interest were further evaluated using qPCR comparing CAC_iv versus CD14⁺ monocytic cells. The CAC_iv transcriptome strongly related to tissue macrophages, and more specifically to regulatory M2c macrophages. The cytokine expression profile of CAC_iv was predominantly immune modulatory and resembled the cytokine expression of tumor-associated macrophages (TAM). Path

SUBMITTER: Everaert BR 

PROVIDER: S-EPMC6525806 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature