Development of fibrocartilage layers in the anterior cruciate ligament insertion in rabbits.

ABSTRACT:

Background

A detailed evaluation focusing on the fibrocartilage layers in the anterior cruciate ligament (ACL) insertion is necessary to consider regeneration of the insertion. This study examined the development of the fibrocartilage layers in the ACL tibial insertion in rabbits by quantitative morphometric evaluations based on histological and immunohistochemical analyses.

Methods

Male Japanese white rabbits were used because of their history of use for histomorphometric analyses of the ACL insertion and to eliminate the influence of female hormones on the ACL. Six animals were euthanized at each age (1 day and 1, 2, 4, 6, 8, 12, and 24 weeks); in total, 48 animals were used. Proliferation rate, apoptosis rate, Sox9-positive rate, and chondrocyte number were evaluated. Saf

SUBMITTER: Mutsuzaki H 

PROVIDER: S-EPMC6525973 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature