A self-assembled, modular nucleic acid-based nanoscaffold for multivalent theranostic medicine.

ABSTRACT: Rationale: Within the field of personalized medicine there is an increasing focus on designing flexible, multifunctional drug delivery systems that combine high efficacy with minimal side effects, by tailoring treatment to the individual. Methods: We synthesized a chemically stabilized ~4 nm nucleic acid nanoscaffold, and characterized its assembly, stability and functional properties in vitro and in vivo. We tested its flexibility towards multifunctionalization by conjugating various biomolecules to the four modules of the system. The pharmacokinetics, targeting capability and bioimaging properties of the structure were investigated in mice. The role of avidity in targeted liver cell internalization was investigated by flow cytometry, confocal microscopy and

SUBMITTER: Andersen VL 

PROVIDER: S-EPMC6525989 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature