In-depth serum proteomics reveals biomarkers of psoriasis severity and response to traditional Chinese medicine.

ABSTRACT: Serum and plasma contain abundant biological information that reflect the body's physiological and pathological conditions and are therefore a valuable sample type for disease biomarkers. However, comprehensive profiling of the serological proteome is challenging due to the wide range of protein concentrations in serum. Methods: To address this challenge, we developed a novel in-depth serum proteomics platform capable of analyzing the serum proteome across ~10 orders or magnitude by combining data obtained from Data Independent Acquisition Mass Spectrometry (DIA-MS) and customizable antibody microarrays. Results: Using psoriasis as a proof-of-concept disease model, we screened 50 serum proteomes from healthy controls and psoriasis patients before and after treatment with trad

SUBMITTER: Xu M 

PROVIDER: S-EPMC6526001 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature