Embryonic cell migratory capacity is impaired upon exposure to glucose in vivo and in vitro.

ABSTRACT:

Background

Impairments in cell migration during vertebrate gastrulation lead to structural birth defects, such as heart defects and neural tube defects. These defects are more frequent in progeny from diabetic pregnancies, and we have recently provided evidence that maternal diabetes leads to impaired migration of embryonic mesodermal cells in a mouse model of diabetic pregnancy.

Methods

We here report the isolation of primary cell lines from normal and diabetes-exposed embryos of the nonobese diabetic mouse strain, and characterization of their energy metabolism and expression of nutrient transporter genes by quantitative real-time PCR.

Results

Expression levels of several genes in the glucose transporter and fatty acid transporter gene families were altered in diabe

SUBMITTER: Herion NJ 

PROVIDER: S-EPMC6526088 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature