Long-fragment targeted capture for long-read sequencing of plastomes.

ABSTRACT:

Premise

Third-generation sequencing methods generate significantly longer reads than those produced using alternative sequencing methods. This provides increased possibilities for the study of biodiversity, phylogeography, and population genetics. We developed a protocol for in-solution enrichment hybridization capture of long DNA fragments applicable to complete plastid genomes.

Methods and results

The protocol uses cost-effective in-house probes developed via long-range PCR and was used in six non-model monocot species (Poaceae: African rice, pearl millet, fonio; and three palm species). DNA was extracted from fresh and silica gel-dried leaves. Our protocol successfully captured long-read plastome fragments (3151 bp median on average), with an enrichment rate ranging from

SUBMITTER: Bethune K 

PROVIDER: S-EPMC6526642 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature