Isolation and identification of EST-SSR markers in Ixonanthes chinensis (Ixonanthaceae).

ABSTRACT:

Premise

Ixonanthes (Ixonanthaceae) consists of between three and 19 species, among which I. chinensis and I. khasiana are considered vulnerable. Here, 58 microsatellite markers were developed for further conservation of these two Ixonanthes species.

Methods and results

RNA transcripts of I. chinensis were sequenced and assembled into 19,545 unigenes, and 994 simple sequence repeat (SSR) loci were identified from 920 contigs. Based on these, 106 primer pairs were designed, 58 were successfully amplified, and 12 demonstrated polymorphism among five populations. The number of alleles per locus varied from three to 10, and the levels of observed and expected heterozygosity ranged from 0.000 to 1.000 and 0.000 to 0.844, respectively. Further ass

SUBMITTER: Guo W 

PROVIDER: S-EPMC6526656 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature