Myocardial differentiation is dependent upon endocardial signaling during early cardiogenesis <i>in vitro</i>.

ABSTRACT: The endocardium interacts with the myocardium to promote proliferation and morphogenesis during the later stages of heart development. However, the role of the endocardium in early cardiac ontogeny remains under-explored. Given the shared origin, subsequent juxtaposition, and essential cell-cell interactions of endocardial and myocardial cells throughout heart development, we hypothesized that paracrine signaling from the endocardium to the myocardium is crucial for initiating early differentiation of myocardial cells. To test this, we generated an in vitro, endocardial-specific ablation model using the diphtheria toxin receptor under the regulatory elements of the Nfatc1 genomic locus (NFATc1-DTR). Early treatment of NFATc1-DTR mouse embryoid bodies with

SUBMITTER: Saint-Jean L 

PROVIDER: S-EPMC6526719 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature