Human red and green cone opsins are O-glycosylated at an N-terminal Ser/Thr-rich domain conserved in vertebrates.

ABSTRACT: There are fundamental differences in the structures of outer segments between rod and cone photoreceptor cells in the vertebrate retina. Visual pigments are the only essential membrane proteins that differ between rod and cone outer segments, making it likely that they contribute to these structural differences. Human rhodopsin is N-glycosylated on Asn² and Asn¹⁵, whereas human (h) red and green cone opsins (hOPSR and hOPSG, respectively) are N-glycosylated at Asn³⁴ Here, utilizing a monoclonal antibody (7G8 mAB), we demonstrate that hOPSR and hOPSG from human retina also are O-glycosylated with full occupancy. We determined that 7G8 mAB recognizes the N-terminal sequence ²¹DSTQSSIF²⁸ of hOPSR and hOPSG from extrac

SUBMITTER: Salom D 

PROVIDER: S-EPMC6527158 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature