A novel combination of chemotherapy and immunotherapy controls tumor growth in mice with a human immune system.

ABSTRACT: Mice reconstituted with a human immune system and bearing human tumors represent a promising model for developing novel cancer immunotherapies. Here, we used mass cytometry and multi-parametric flow cytometry to characterize human leukocytes infiltrating a human breast cancer tumor model in immunocompromised NOD.SCID.γc-null mice reconstituted with a human immune system and compared it to samples of breast cancer patients. We observed highly activated human CD4⁺ and CD8⁺ T cells in the tumor, as well as minor subsets of innate immune cells in both settings. We also report that ICOS⁺ CD4⁺ regulatory T cells (Treg) were enriched in the tumor relative to the periphery in humanized mice and patients, providing a target to affect Treg and tumor growth

SUBMITTER: Burlion A 

PROVIDER: S-EPMC6527285 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature