In vitro differentiation of single donor derived human dental mesenchymal stem cells into pancreatic β cell-like cells.

ABSTRACT: The present study was carried out to investigate and compare the in vitro differentiation potential of mesenchymal stem cells (MSCs) isolated from human dental tissues (pulp, papilla, and follicle) of the same donor. MSCs were isolated from dental tissues (pulp, papilla, and follicle) following digestion method and were analyzed for the expression of pluripotent markers and cell surface markers. All three types of MSCs were evaluated for their potential to differentiate into mesenchymal lineages. Further, the MSCs were differentiated into pancreatic β cell-like cells using multistep protocol and characterized for the expression of pancreatic lineage specific markers. Functional properties of differentiated pancreatic β cell-like cells were assessed by dithizone staining and glucose

SUBMITTER: Shivakumar SB 

PROVIDER: S-EPMC6527933 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature