Core Catalysis of the Reductive Glycine Pathway Demonstrated in Yeast.

ABSTRACT: One-carbon (C1) compounds are attractive microbial feedstocks as they can be efficiently produced from widely available resources. Formate, in particular, represents a promising growth substrate, as it can be generated from electrochemical reduction of CO₂ and fed to microorganisms in a soluble form. We previously identified the synthetic reductive glycine pathway as the most efficient route for aerobic growth on formate. We further demonstrated pathway activity in Escherichia coli after expression of both native and foreign genes. Here, we explore whether the reductive glycine pathway could be established in a model microorganism using only native enzymes. We used the yeast Saccharomyces cerevisiae as host and show that overexpression of only endogenous enzymes enables glycine

SUBMITTER: Gonzalez de la Cruz J 

PROVIDER: S-EPMC6528164 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature