R-spondin2 promotes hematopoietic differentiation of human pluripotent stem cells by activating TGF beta signaling.

ABSTRACT:

Background

Human pluripotent stem cells (hPSCs) provide supplies of potential functional blood cells to suffice the clinical needs. However, the underlying mechanism of generating genuine hematopoietic stem cells (HSCs) and functional blood cells from hPSCs remains largely elusive.

Method

In this study, we supplied R-spondin2 exogenously during hematopoietic differentiation of hPSCs under various culture conditions and analyzed the production of hematopoietic progenitor cells (HPCs). We further added R-spondin2 at different temporal window to pin down the stage at which R-spondin2 conferred its effects. RNA-SEQ-based gene profiling was applied to analyze genes with significantly altered expression and altered signaling pathways. Finally, megakaryocytic differentiation and pl

SUBMITTER: Wang Y 

PROVIDER: S-EPMC6528258 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature