Investigating function roles of hypothetical proteins encoded by the Mycobacterium tuberculosis H37Rv genome.

ABSTRACT:

Background

Mycobacterium tuberculosis (MTB) is a common bacterium causing tuberculosis and remains a major pathogen for mortality. Although the MTB genome has been extensively explored for two decades, the functions of 27% (1051/3906) of encoded proteins have yet to be determined and these proteins are annotated as hypothetical proteins.

Methods

We assigned functions to these hypothetical proteins using SSEalign, a newly designed algorithm utilizing structural information. A set of rigorous criteria was applied to these annotations in order to examine whether they were supported by each parameter. Virulence factors and potential drug targets were also screened among the annotated proteins.

Results

For 78% (823/1051) of the hypothetical proteins, we could identify homo

SUBMITTER: Yang Z 

PROVIDER: S-EPMC6528289 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature