Oxidative stress mediates depot-specific functional differences of human adipose-derived stem cells.

ABSTRACT: BACKGROUND:Visceral (VS) fat depot is known to have defective adipogenic functions compared to subcutaneous (SC) fat, but its mechanism of origin is unclear. OBJECTIVE:We tested our hypothesis that the degree of oxidative stress in adipose-derived stem cells (ASCs) from these depots may account for this difference. METHODS:ASCs were isolated from VS (omental region) and SC (abdominal region) fat depots of human subjects undergoing bariatric surgery. ASCs from VS and SC fat were investigated for their cellular characteristics in reactive oxygen species (ROS), metabolism, gene expression, proliferation, senescence, migration, and adipocyte differentiation. ASCs were also treated with antioxidant ascorbic acid (vitamin C). RESULTS:We found that human VS-derived ASCs exhibit excessive oxidativ

SUBMITTER: Sriram S 

PROVIDER: S-EPMC6528291 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature