Culturing marine bacteria from the genus Pseudoalteromonas on a cotton scaffold alters secondary metabolite production.

ABSTRACT: The discovery of secondary metabolites from marine microorganisms is beset by numerous challenges including difficulties cultivating and subsequently eliciting expression of biosynthetic genes from marine microbes in the laboratory. In this paper, we describe a method of culturing three species from the marine bacterial genus Pseudoalteromonas using cotton scaffold supplemented liquid media. This simple cultivation method was designed to mimic the natural behavior of some members of the genus wherein they form epibiotic/symbiotic associations with higher organisms such as sponges and corals or attach to solid structures as a biofilm. Our scaffolded cultivation is highly effective at stimulating an attachment/biofilm phenotype and causes large changes to metabolite profiles for the microbes

SUBMITTER: Timmermans ML 

PROVIDER: S-EPMC6528606 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature