Cocaine promotes primary human astrocyte proliferation via JNK-dependent up-regulation of cyclin A2.

ABSTRACT:

Purpose

Astrocytes perform a plethora of important functions in the central nervous system (CNS) and are involved in cocaine-evoked synaptic plasticity. Previously, we showed that while cocaine decreased cyclin A2 expression in primary human neural progenitor cells, it increased cyclin A2 expression in human astrocytes. Since cyclin A2 is an essential regulator of the cell cycle, the aim of the present study is to clarify the effect of cocaine on proliferation of human astrocytes and elucidate the underlying molecular mechanisms.

Methods

Primary human astrocytes were treated with either 1, 10, or 100 μM cocaine for 48 hr, and cell proliferation was measured using the CyQUANT cell proliferation assay. To elucidate the molecular mechanisms through which cocaine affects the pro

SUBMITTER: Lee CT 

PROVIDER: S-EPMC6528657 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature