BMAL1 deficiency promotes skeletal mandibular hypoplasia via OPG downregulation.

ABSTRACT:

Objectives

Skeletal mandibular hypoplasia (SMH), a common type of developmental deformities, results in impaired aesthetics of facial profile, occlusal dysfunction and poor life quality. In this study, BMAL1 deficiency leads to SMH formation, and we aim to investigate the mechanism by which BMAL1 deficiency induces SMH.

Materials and methods

Circadian rhythm-disordered mouse models were constructed by placing animals in a jet lag schedule of 6-h light advance every 7 days for 4 or 8 weeks. The OPG expression was evaluated by histomorphometry, immunohistochemistry and western blot analysis. The mechanism by which BMAL1 affects OPG expression was investigated by chromatin immunoprecipitation and luciferase reporter assays. The phenotypes caused by BMAL1 knockout can be rescued

SUBMITTER: Zhou X 

PROVIDER: S-EPMC6528896 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature